BIO203 Official Handouts

EXTENSION

 The temperature at this step depends on the type of DNA polymerase used.  Taq polymerase has its optimum activity temperature at 7080°C.  Commonly 72°C is used.

EXTENSION

 At this step the DNA

synthesizes a new DNA strand complementary to the DNA template strand by adding dNTP's.

FINAL ELONGATION

 Single step is performed at a temperature of 7074°C for 5-15 minutes after the last PCR cycle to ensure that any remaining single-stranded DNA is fully extended.

FINAL HOLD TEMPERATURE

 This step at 4-15°C for an indefinite time may be employed for short-term storage of