BIO203 Midterm MCQs by Amaan Khan

BIO 203-Midterm

M.C.Q

1. _________________ an enzyme derived from the bacterium Thermus aquaticus. Taq polymerase
2. PCR can be performed to amplify________________________________ DNA.
3. The denaturation temperature ranges from____________ 93°C - 98°C
4. During extension the most common temperature is ______________ 72°C
5. Which step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTP's? Extension
6. The thermocycler works on the principle of __________which raises and lowers the temperature of the block in a pre-programmed manner. Peltier effect
7. Primers are single stranded ___________ bp long DNA fragments. 18–30
8. Which one of the following determine the specificity of the PCR reaction? Primer
9. FORMULA FOR CALCULATING MELTING TEMPERATURE OF PRIMERS. Tm = 4(G+C) + 2(A+T)
10. The extension rate of Taq polymerase____________ 75 nt/sec
11. Which one of the following is good or perfectly matched primers between 19-24 nt? 94-55-72
12. Misprimes may occur due to non-specific_______________ of primers. Hybridization
13. Controls for contamination contains all reagents except DNA template is called________ Blank reaction
14. Two pairs (instead of one pair) of PCR primers are used to amplify a fragment______ NESTED PCR
15. Which type of PCR has Very low probability of nonspecific amplification? NESTED PCR
16. ___________ is a variant of PCR which enables simultaneous amplification of many targets of interest in one reaction by using more than one pair of primers. Multiplex PCR

Q17. Which temperatures for each of the primer sets must be optimized to work correctly within a single reaction?

Annealing

18. Amplicon sizes should be different enough to form distinct bands when visualized by_____ gel electrophoresis
19. The enzyme used to convert RNA into cDNA____________ Reverse transcriptase
20. Which types of PCR is widely used in expression profiling, to determine the expression of a gene? RT-PCR
21. _________ is used to measure the quantity of a PCR product. Real Time PCR
22. Which is a technique that reduces nonspecific amplification during the initial set up stages of the PCR? Hot

start PCR

23. Allele-specific PCR used for identifying of_________________________ SNPs
24. In Situ PCR (ISH ) is a polymerase chain reaction that actually takes place inside the cell on a slide.
25. Flanking DNA sequences are digested and then ligated to generate______________ Circular DNA
26. A method used to allow PCR when only one internal sequence is known___________ Inverse PCR
27. AFLP stands for________________ Amplified Fragment Length Polymorphism
28. ___________ is a highly sensitive PCR-based method for detecting, polymorphisms in DNA. AFLP
29. ________ is a group of procedures that allow amplification to occur at many locations in a genome. Whole

genome amplification

30. In Mini Primer PCR the thermostable enzyme extend primer consist ________ nucleotides. 9 or 10
31. PCR has been used in gene cloning and screening of___________________ Genomic libraries
32. __________ is the application of scientific procedures to solve criminal and legal matters. Forensic Science
33. ___________ is a forensic technique used to identify individuals by characteristics of their DNA. DNA