BIO203 Grand Quiz by Team VU Legends

GRAND QUIZ · BIO203 LATURE 1-35 · EFFORT BY TEAM OF VU LEGENDS · ADMIN: JIYYA RANA

1. .............. is a technique widely used in molecular biology and biotechnology. PCR
2. ............. name is from one of its key component DNA polymerase. PCR
3. As PCR progresses .......... generated is itself used as template for replication. DNA
4. During .......... a heat stable DNA stable polymerase, such as Taq polymerase an enzyme derived from the becterium ............. PCR , Thermus Aquaticus
5. PCR is extensively modified to perfome a wide array of ............. Genetic manipulation

Q6. How many steps of PCR?

7. Name three steps of PCR Denaturation Annealing Extension
8. Reaction is heat at the temperature of ............ which held for 1-10 minutes. 93°C - 96°C
9. The reaction temperature is lowered to ........... for 20 -40 seconds. 40-65°C
10. Taq polymerase has its optimum activity temperature at........... 70-80°C
11. Common temperature for the Taq polymerase is.............. 72°C
12. At which step DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTP's. At extension
13. Single step is performed at a temperature of ........... for 5-15 minutes. 70-74°C
14. Write the name of major steps of PCR.

Thermal cycler DNA tamplate Primers Buffer MgCl2 Distilled water Deoxynucleotide tryphisphates DNA polymerase

15. .............. are small cylindrical plastic tube with conial buttoms.
16. ............ works on the principal of peltier effect which raises and lower the temperature of the block in a pre-progremmed manner Thermocycler
17. Taq , Pfu , Vent polymerase are the ............... DNA polymerase
18. Primers are single stranded ........... long DNA fragment. 18-50 bp
19. ............ determine the specificity of the PCR reaction. Primers
20. Distance between the primer binding sites will determine the ........... of PCR products. Size
21. Product size of primers is ........... 100 -700 bp
22. Write the formula for calculating melting temperature of primers. Tm = 4(G+C) + 2(A+T)