BIO505T Final Term Objective Guess Paper 2023

BIO-301 MCQs FINAL TERM- 100% · GUESS PAPER 2023 · Essentials of Genetics · BY SULMAN ALI · OBJECTIVES

•  Double mutation has a fitter phenotype than either single mutation.
•  Proteins are made of "amino acids"
•  Nucleotides contain "ribose sugar"
•  Sequence of development “haploid cells diploid zygote"
•  Diploid number is obtained after "fertilization"
•  Colichine produces "polyploidy"
•  The uses of vntr "geomic mapping etc"
•  RNA is obtained from"cytoplasm"
•  Northern blot is used for *RNA analysis*.
•  The following is not chromosomal aberration is *mitosis*.
•  R banding has dark bands of *adenine and thymine content*.
•  Cancer and infertility are the result of *genetic mutations*.
•  Colchicine is used to *promote polyploidy*.
•  A cross for determination of the genotype of an individual is called a *test cross*.
•  Autosomal dominant diseases are caused by *dominant alleles on autosomal
•  Mitochondrial DNA* is inherited through ovum, not sperm.
•  Sequence difference compared to a reference standard present in at least 1-2% of a population is called *polymorphism*.
•  Which on is sexual disease ____________________________Alzheimer's disease.
•  Discontinuous variations are entirely genetically controlled.
•  forward genetics is defined as____ The identification of genes responsible for a specific phenotype without prior knowledge of the gene's function.
•  genetic marker for a single locus is _______ RFLP, Microsatellite, STS.
•  in x-linked recessive trait all are true except__ "In X-linked recessive traits, males

can be carriers of the trait."/ it is present in females more than males

 a healthy male and female have a sòn who is color blind .what is genetics of boy parents?

XcY

•  Dark bands are those which contain fewer or less intense light waves, and light bands are those which contain more or more intense light waves.
•  The chromosome number in an earthworm is 2.

likely genotypes of the boy's parents?

•  Boy = XaY (got recessive allele from mother) He will be color blind.
•  Position depended method of disease identification following _______Reverse

genetics

•  Molecular biology, forensic , genes mapping are application of ________________VNTR
•  Groups of human chromosomes are __________________________7
•  Dna precipitation k liye konsa chemical use hota?___Isopropanol
•  Important reagents for pcr. Option mai sb hi mojood thy tu _____ALL
•  Mitochondrial inheritance ki cgaracteristics di thi option mai. Ghalat konsi ye btana tha. These are present chose other than these (⚫ Heteroplasy ⚫ Variable expression ⚫ Pleiotropy ⚫ Reduced penetrance ⚫Functional somatic mosaic).
•  Q-PCR is commonly used to determine whether a DNA sequence is present in a sample and the number of its copies in the sample.
•  Meiosis I ki characteristics pochi thi option mai. Ye b ALL
•  In which pcr two pairs of primers are used.______Nested PCR
•  In giemsa staining dark bands are rich in... A and T ans
•  And light bands are rich in.... G and C ans
•  During rna extraction, rna is extracted from the neucli of which cell. Rbc, wbc, or platelets
•  Erythrocytes k bry mak 1 mcqs tha______ Erythrocytes (red blood cells) do not contain nuclei. They neither synthesize nor contain RNA.
•  Which fur color of rabbit have highest numbers of genotypes. Grey
•  Which genetic disorder have lowest frequency. Ans autosomal recessive disorder
•  Which is an autosomal recessive disorder? (⚫ Bloom syndrome ⚫ Carpenter

syndrome ⚫ Cystic fibrosis ⚫ Thalasemia ⚫Many forms of mental retardation)

•  Which mutation shortens the protein? Ans non-sense mutation
•  How many cases of turner syndrome are due to ring chromosome. Ans 20%
•  During inversion what is reversed.___chromosome sequence/segments of
•  Which mutation suppreses the effect of other mutation. Ans suppresion mutation
•  DOMINANT EPISTASIS: Epistatic gene exerts its affect with the presence of a dominant allele.
•  Mutation that occur in regulatory region affects? Ans transcription, Translation

and splicing of protein

•  AUTOSOMAL DOMINANT DISEASES (⚫ Marfan syndrome ⚫ Huntington's disease ⚫ Retinoblastoma ⚫Waardenburg syndrome ⚫Myotonic dystrophy ⚫ Polycystic kidney disease ⚫ Achondroplasia ⚫ Polydactyly ⚫ Heredity hearing loss)
•  X-LINKED DOMINANT DISEASES: (⚫ Rett syndrome ⚫ Alport syndrome ⚫ Goltz syndrome ⚫ Fragile-X Syndrome)
•  Homologous paired chromosomes have the same shape, size, and location of the centromere.
•  in organic method of purification of DNA ...... IS USED TO remove protein (ans)

phenol chloroform

•  ..... Is chelating agent(ans)EDTA
•  ..... Result in two cells(ans) mitosis
•  application thi VNTR ki uska answer bh VNTR hi th
•  R banding has dark bands of: (ans) Heterochromatic
•  Fur color in labrador retriever controlled by….(ans)2gene
•  Two pairs of primer use in…(ans) nested PCR

probability that any son born will have pattern baldness?.. (ans)50%

•  A mutation that are responsible for shorten of protein due to producing stop

codons….(ans)non sense mutation

•  when allele of two gene segregate together and transmitted as a unit? What type of segregation occur... (Ans) Dependent segregation
•  dna fingerprinting is an example of??VnTR
•  If normal male and normal female are not color blind but their son is color blind what are the alleles of next generation: A) Son carry recessive allele
•  Hemophilia is an example of____X linked chromosome.
•  Two pairs of primer use in….. nested pcr
•  Genes in drosophila.......13,700
•  A mutation that are responsible for shorten of protein.......... Nonsense mutation
•  ............. Caused frameshift mutation._______ Deletion
•  First stage of mitosis.......Prophase
•  First phase of mitosis........Prophase
•  ............... is non-ionic buffer for DNA extraction ( cell lysis buffer)
•  If both parents have heterozygous dominant disease, what will be the chance of a child not having that disease? (25%)
•  microbiology, finger printing, genetic diversity and mapping of genomes are applications of........VNTR.
•  Symbol of substitution ____ ">"
•  RNA is genetic material in.....some viruses
•  A type of genetic study in which , select a gene create a mutation then study its phenotype ............. Forward genetics.
•  If two people who are both carrier for the genetically inherited fatal recessive disease decide to become parents , how many chances that their children will also be carriers.( 1

out of 4)

•  Any change in nucleotide sequence of DNA gene is called......mutation
•  Which disorder have the lowest frequency range.......X linked disorder.
•  In phenol- chloroform DNA extraction lower organic phase contains........lipids
•  DNA finger printing bioinformatics are applications of ( ans)VNTR
•  R banding has dark bands of: ( ans) Heterochromatic
•  Two pairs of primer use in…( ans) nested PCR
•  A mutation that are responsible for shorten of protein due to producing stop codons….(