BT102 Midterm Past Papers Objective

Which of the following contains mycolic acid?

Mycobacterium & Nocordia · After gram staining, gram positive bacteria appear: · Purple (crystal violet) · Rod shaped bacteria are called: · Bacilli · Energy is required in: · Active transport

Which of the following structure is involved in the locomotion of bacteria?

Flagella · Gram positive bacteria produced: · Exotoxins

The basal body of flagellum of gram-positive bacteria consist of … rings.

70s ribosome consist of two subunits:

50s & 30s

… are used for twitching motility.

Pili

Integral proteins are … in the cell membrane and are … in water.

Imbedded, insoluble

Which of the following pH is acidic?

All

(acidity < 7)

Bacteria get nitrogen from?

All

(Decomposing proteins, NH4+ ions, nitrates, gaseous N2, nitrogen fixation) Which of the following is the characteristic of coenzyme?

All

(Act as electron carriers + receive electrons from nutrients + donate electrons to other molecules in subsequent reactions) Bacteria get sulfur from?

All

(Sulfate ions, hydrogen sulfide, sulfur containing amino acids)

Which of the following is the physical source/requirement of growth?

All

(Temperature + pH + osmotic pressure)

Which of the following bacteria formed endospore?

All

(Clostridium botulinum, bacillus cereus, bacillus anthracis, bacillus thuringiensis, clostridium tetani) In anaerobic respiration, final electron acceptor is inorganic substance other than O2 is:

All

(Nitrate ion, nitrogen fixing bact, sulfate ion, H2S forming bact, carbonate ion CH4 forming bact) Which of the following is required for aerobic respiration?

None of the given option Psychrophiles are extremophilic organisms that are capable of growth and reproduction in temperature around:

-20°C & +10°C · O2 has no effect on: · Aerotolerant aerobes

Which of the following is not present in bacteria?

Both histone & nuclear membrane Bacteria get phosphorus from:

Phosphate ions · Bacteria utilize oxygen for: · Aerobic respiration · Specialized resting cells: · Endospores

Which of the following is used to maintain the desired pH?

Buffer

Photoautotrophs use … as a source of energy.

Light

NADH & FADH2 during Kreb′s cycle oxidized via ETC & generate ATP molecules:

36 or 38

Bacteria need … for the synthesis of protein & nucleic acids.

Nitrogen

Which of the following compounds are needed for nucleic acid synthesis?

Sulfur & phosphorus

Which of the following group of microbes strictly need oxygen for growth?

Obligate aerobes

Which of the following enzymes catalyze the conversion of atmospheric oxygen into hydrogen peroxide?

Superoxide dismutase

Which of the following is hyperthermophile in nature?

Archaea · Removal & gain of electrons from an atom: · Redox reaction · Helicobacter pylori is a: · Aerotolerant aerobe · Movement against the concentration gradient: · Active movement

Biological oxidations involve the loss of hydrogen atoms they are also called … reactions.

Dehydrogenation · All organic compounds have: · Carbon

Cytoplasm contains … percent water.

80% · Cold loving bacteria: · Psychrophiles · Chemoheterotrophs use carbon from: · Proteins, carbohydrates, lipids Removal of electrons: · Oxidation · Aerobes that require O2 in low amounts are: · Microaerophiles

Besides water, … is the most important requirement for bacterial growth.

Carbon

Oxidation of glucose to pyruvic acid with the production of some ATP and energy-containing NADH is known as:

Glycolysis · Microorganisms that use light as a source of energy: · Photoautotrophs

Which of the following is used for catalase test?

Hydrogen peroxide · Which of the following is anoxygenic: · Rhodopseudomonas Chemoautotrophs use carbon from: · CO2

Which of the following condition in which water moves into the cell?

Hypotonic

NADH & FADH are oxidized during:

ETC (Electron transport chain) Which of the following initiate the spore activation?

Heating · Facilitated diffusion is done with the help of: · Integral proteins · Kingdom Monera contains: · Bacteria & Archea · Slide or specimen is placed on: · Stage of microscope

Which is used for mordant in gram staining?

Iodine · The primary stain is used in acid fast staining is: · Carbol fuchsin · Syphilis is caused by: · Treponema pallidum

Which is antigenic in nature?

1. side chain (teichoic acid in gram + bacteria) Which is used to transform DNA from bacteria to another?

Pili

… is also called volutin.

Metachromatic granules · The optimal temperature for growth of hyperthermophiles is: · 80°C · Which one is indicated by clear colorless zone surrounding: · Β-hemolysis · Do not contain cell wall: · Mycoplasmas