BT301 Grand Quiz MCQs by Amaan Khan

BT 301 M.C.Q Grand Quiz

i. DNA is amplified by the technique is called ________ PCR ii. How many types of nucleotides are present in DNA? 4 iii. LTR (Long terminal repeat) consists of ______ 250 – 600 bp iv. In cloning technique a plasmid with low molecular weight and high copy number is more suitable. v. The number and size of the fragments generated by the restriction enzyme depends on the frequency of occurrence of the target site in the DNA to cut. vi. Who gave the most important clue about the structure of DNA before 1953? Chargaff vii. The bacterial chromosome is very long ______ up to 1mm viii. By removing the cell wall from the bacteria the remaining cell is called ______ protoplast ix. Nuclein was discovered in ______ 1869 x. Southern blotting is used for the detection of ______ specific DNA xi. pSC has _______ tetracycline resistance gene. xii. The branch of biotechnology that deals with the production of drugs is called _____ Red Biotechnology xiii. The used of salt of for the preservation of meat __________ 400BC xiv. Most of th useful R-M system is Type II xv. Lambda have ______ 48502 bp xvi. Which one of the following is natural occurring plasmid? Col E1 and pSC 101 xvii. Structural instability is of plasmid may be arise due _______ deletion or rearrangement of DNA xviii. Nucleotide with 14500 bp is minisatellites and microsatellites up to 13 bp xix. The successive bond present between two nucleotides is called_______ Phosodiester linkage xx. The DNA Ligase is repair by _______ Nick

21. Type I system was the first to be characterized by the E. coli K12.
22. Before _________ no rooted of cloning DNA. 1970
23. E. coli often remains of the host of choice for cloning.
24. Most common SINSs in primate are called ________ Alu sequences
25. Mobile DNA moves within ___________ Genome
26. Pseudo genes are ________ copies of gene. Non functional
27. DNA combine with the histone are called _________ Chromatin
28. Which one of the following is a Purine? Adenine and Guanine
29. In Autoradiography _____________ is used to visualize. X-rays film
30. _________refers to the conventional techniques that have been used for many centuries to produce beer, wine,

cheese etc. Conventional Biotechnology

31. In 1829 the Urea is prepared in laboratory? Fredrick Wohler
32. Ti Plasmid is found in _________ tumor
33. Primary structure of protein is also known as _________ covalent structure
34. Retrovirus are used to produced infection __________ cDNA
35. The minor groove of the DNA have _____________ 3.4 A
36. I973 ________ brought the restriction enzyme. Boyer
37. __________ was the VC of Genentech. Robert Swanson
38. Complete genome study of Yeast was completed in ______ 1996
39. Organisms that contains single circular organelle _______ Bacteria
40. A multisubunit protein ______ Quaternary
41. RNA act as a template _____________- mRNA
42. A molecules that consist of three components are called ________ Nucleotide
43. RNA contains _____ sugar. Ribose
44. Schwann proposed cell theory in _______ 1839 AD
45. DNA is visualize by the ________ X-rays / Autoradiography
46. The process of forming mRNA on a DNA template ___________ transcription
47. Transfer RNA acts as ______ molecule in the protein synthesis process. Adapter
48. _________ ribosome are present in the rRNA. 60%
49. The more extent form of DNA is called _______ Euchromatin
50. Ethidium bromide used as ________ to dye. stain
51. C-N bond is shorter in ________ Protein
52. How many types of Amino acids are present in human body? 20
53. In protein each amino acid are joined by another amino acid by covalent bond is called _____ peptide linkage
54. The six atoms of the peptide group are coplanar i.e., lie in a single plane, with the oxygen atom of the carbonyl group and the hydrogen atom of the amide nitrogen trans to each other.
55. _________ is a more extended conformation of polypeptide chains. B-sheet
56. Western blotting is used for detection of ________ protein
57. Bacteria has high coding density than ________ Eukaryotes
58. In human __________ numbers of genes are present. 23000
59. The overall three dimensional arrangement of all atoms in a protein is referred to as the protein’s tertiary

structure.

59. The percentage of nucleic acid in relation to protein is about 1% for the influenza virus and about 50% for some bacteriophage.
60. bacterial DNA to form a highly condensed nucleoprotein complex called the Nucleiod.
61. Northern blotting used for the ___________ RNA
62. Mitochondrial gene are in human cell _______ 37 genes
63. Highly condensed chromatin is known as heterochromatin.
64. Cell lyses by SDS and proteinase K
65. Agarose is convenient for separating DNA fragments ranging in size from a few hundred base pairs to about 20 kb.
66. Polyacrlamide is preferred for smaller DNA fragments.
67. Probe is a fragment of DNA or RNA which is used to detect the sequences of target DNA.
68. In electroporation, electric field is applied to cells in order to increase permeability of the cell membrane to take DNA.
69. At least four R-M systems are known.
70. Type III enzymes have symmetrical recognition sequences but otherwise resemble type I systems and are of little value.
71. Type II endonucleases recognize and cleave DNA within particular sequences of four to eight nucleotides which have a twofold axis of rotational symmetry i.e. referred as palindromes
72. Nucleases or DNases are the enzymes that degrade DNA.
73. An enzyme that catalyzes template-dependent synthesis of DNA. DNA polymerase
74. An enzyme that creates a phosphodiester bond between the 3’ end of one DNA segment and the 5’ of another.