BT403 Current Objective

*BT 403 Agriculture Biotechnology* *Final paper 18-8-23* collected by work together team

*Mcqs*

1. which possess half the number of chromosomes from a Diploid species.___monoploids
2. It has been estimated that __ is fixed by different legume crops by the microbial activities Of Rhizobium.__40-250 kg N / ha / year
3. Worldwide, there is percent global primary crop production from genetically modified crops,___9%
4. Canola is genetically engineered form was approved in___ 1996,
5. injecting a gene for a rat Growth hormone into a mouse embryo in___ 1982.
6. GMO stand for ___ genetically modified organisms
7. Transformed cells with a single copy of the transgene usually show higher and More stable expression than multicopy lines, in which expression may suffer from Posttranscriptional ___ gene silencing.
8. Rate of transportation ____ in plant with pathogenic attack ___ increase
9. Hormone induce callus formation ___ cytokinin
10. The genetic markers should be_________and______: palymorphic;. Heterazygous:
11. Almost perecent of corn grown in the U.S. is genetically modified.__85%
12. The creation of the first recombinant DNA molecules by Paul Berg in___________ 1972
13. ________ can usefully be defined as the branch of philosophy concerned witecide what is Morally wrong and what is morally right. ___Ethics
14. Glycine is___ acid ___ amino acid
15. For tissue growth in the medium sucrose concentration of ( 2%)
16. Rate of respiration (increases) in diseased plants
17. Blight disease is caused by (chlorosis)
18. In (India) technique of cyanobacteria inoculation to rice field being followed
19. Russian study of rat fed with GMO soy show (five) folds increase in mortality
20. (GM) Tomato can tolerate lengthier shelf life
21. RNA hydrolyzing enzymes ( Such sterile male plants are created by introducing a gene coding for an Enzyme (barnase), which is an RNA hydrolyzing enzyme) that inhibits Pollen formation.)
22. Chilling injuries common in plant Except (Maize)
23. (Ammonium nitrate) Is macronutrient for Murashige and skoog
24. Auxin Favor DNA duplication and Cytokinin enables the separation of (chromosomes

separation)

25. Growth Regulators/ growth promotors hormones (Auxins, Gibberellins, Cytokinin)
26. How pesticides enter our body by (mouth, lungs, skin) (2 times)
27. In 1990 1st food product of biotechnology is (Cheese)
28. Most callus will be initiated cut surface within( 3 to 8 weeks)
29. Heat shock protein size of HSP100 class is (100 to 114 Kda)
30. Which cause ripening (ethylene)
31. How many phases of regeneration? (Three)
32. Multiple form of same enzymes coded by different genes (Isozymes)
33. Powdery mildew is (fungal disease)
34. Commonly transformation occur in (bacteria)
35. ROS is not found in….(The ROS production in plants is mainly localized in the chloroplast, Mitochondria and peroxisomes.) ..cell wall ans
36. Late blight of potato disease caused by (phytopthora infestants)
37. Plastid genome ______
38. Light stress is __a-biotic
39. Incorporation of genes from one to another _Transformation__
40. Herbiside used where need _and kill to weeds__
41. .Types of genration _two__
42. Which is firstly used for genetic technique Bacteria____
43. Which is work at moderate temperature and help in cells formation ___
44. Vessels are_glasswares___
45. .Ozone cause all of these
46. Optimum temperature for culture media _22 to 28 degree C__
47. Apoenzyme are……..the protein part of an enzyme
48. symptom of pesticides which are not for short time……
49. vessels used for culture ( lead to prevention water & mineral Translocation)petri plates, test tubes, “Magenta boxes,” bottles, and flasks…
50. herbicide are…….chemicals used to kill weeds.
51. Tumer causing bacteria……Helicobacter pylori
52. . FR13A rice-type has high level flooding Tolerance

53. Anthesis is? Anthesis is the first opening of a flower during process of flowering

Subjective questions

1- Enlist the pathogenesis related Pr proteins??marks 5

2Difference between infrastructural constraints and financial constraints. Marks 5 3Role of Cytokinesis on plants?marks 5

4- What are the growth characteristics of cell suspension?? 3

5- What are the environmental effects of pesticides?? 3

6- What are the precautions to use Murashige and Skoog medium?? 3

7- Method by which plants become stress tolerant?? 3 · 8- Nutritional value of genetic engineering? 3 · 9- Role of bio fertilizer? 3 · 10- Steps of initation and maintenance in callus medium? 3 · 11- Sever 6 symptoms of pesticides poisoning? 3 · 12- Drawbacks of pesticides on environment? 5 · 13- Advantage of GM Crops? 5 · 14- Method of regeneration? 5 · 15- Mode of action of herbicides ? 5 · 16- .Step wise tissue culturing ? 5 · 17- .name some glass wares used in tissue culture ? 5 · 18- effects of heat stress in plants ? 5 · 19- .How to treat herbicide burnt skin area? 3

20- How synthetic seeds are beneficial over natural seeds? 3

21- how heat stress effects the photosynthesis? 3

22- .stratgies of pathogens attack ? 3

23- . Zygote related tha ak ?

24- Name the physical response against abiotic factors? 5 · 25- Draw back genetically modified organisms? 5 · 26- Why herbicide are use? 5 · 27- Charcoal affect the anther and pollen culture? 5 · 28- Physical response against abiotic factors? 3 · 29- Use of herbicide? 3 · 30- Bio pesticide? 3 · 31- Stress resistance mechanism? 3 · 32- Why plant virus use transfer gene? 3 · 33- Development in plants ? 3 · 34- What is genome editing? 3 · 35- Morphogenesis and enlist it’s two types? 3 · 36- Why nitrogen fixation is necessary for plants? 3 · 37- 6 symptoms of severe poisoning of pesticides? 3 · 38- What is the use ca MV in gene transfer?5

39- How genetic engineering practice affect the environment?5

40- Write a note on cytokinin on plants?5 · 41- What are phenolic compounds? 5 marks · 42- What is role of auxin in plants? 5

43- How genetic engineering practice affect the environment? 5

44- What are advantages of herbicide? 5 · 45- What is BT cotton? 3 · 46- What is post mitotic anthers? 3 · 47- What are botanical pesticides? 3 · 48- Why pesticides affect childs more than adults? 3