BT503 Grand Quiz 107 MCQs

1. freshwater species Chlamydomonas reinhardtii was sensitive to ______________________________all
2. which of the following factors contribute to the increase in the death rate of the pathogenic microorganisms _____________________________________________ high DO concentrations and an increase in pH
3. Plants are susceptible to bacterial pathogens such as Erwinia carotovora, which produces enzymes capable of degrading its _________________________________________________________cell walls.
4. The average oceanic temperature is around ____________________________________________1–3°C
5. Which of the following bacteria causes crown gall disease ______________Agrobacterium tumefaciens
6. Which zone is essential in the treatment line for biological removal of phosphorus ____ both of the above
7. Salt tolerance in tomatoes has been established by introducing genes involved in …..antiport, the transport of sodium and hydrogen ions in opposite directions across a membrane______ __hydrogen and sodium
8. Those species which live in higher Kcl levels are known a____________ extremophiles __________________ halophile
9. The process of expression of foreign genes in a plant is called ___________________ Gene expression
10. The removal of pollutants by plant that breaking down into compounds that can be vaporized is known as --

-----. Degradation, phytovolatislation, phytosorption

11. The removal of the organic matter in the secondary stage is carried out through biochemical reactions undertaken by. __________________________________________________________ microorganisms
12. Enzyme used in PCR is ….___________________________-_____________________ DNA Polymerase
13. in facultative aerated lagoons are oxygen is supplied by mechanical equipment called aerators._______________________________________ _______________________________aerators
14. denaturation step in PCR usually occur at which temperature? _____________________________ 95 ◦C
15. Is thermophilic microorganism from with polymerase has been isolated and used for PCR purpose._____________________________________________________________ Thermus aquaticus
16. many planktons at an increased rate in lakes when there are high centralization of __ BOTH of the above .
17. in bacteria the most likely candidates for genetic transfer are..____________________ both of the above
18. the first working draft of the human genome sequence was published in which year ?____________ 2001
19. which of the following is considered as health threatening microorganism of water ____________________________________________________ Vancomycin-Resistant Enterococcus
20. Halophiles are extremely important in environment biotechnology these are microorganisms that survive in

highly saline environment . they do so by ensuring that---_______________________________________-

-. Their cytoplasm contains a higher concentration of solutes then their surroundings

21. DNA is precipitated by __________________________________________________________ Alcohol
22. Genomic library are very useful to look for a specific Sequence of __________________________ DNA
23. Typically the soil types best suited to biotechnological interventions are ____________sands and gravels
24. Which kind of porous medium is used in surface flow wetland____________

Q25. Which of the following chemicals is involved in the detoxification of the metals?

26. which of the following contaminants are rich in trichopria tetrat _______________________ Phosphorus
27. During nitrification PH level ?________________

Q28. Zooplanktons are microscopic ?

29. Wolffia globosa is an important tool for showing ….. sensitivity and contamination._________ cadmium
30. Multicellular organism cannot tolerate temperature….__________________________________ 50 ◦ C
31. In eukaryotes the first product of transcription form DNA is …

Grand Quiz · BT503 Environment Biotechnology · 30/ MARKS · (in lieu of Midterm examination) · 1 to 117 lessons for grand quiz · 107 MCQs

32. Bacillus thuringiensis tends to use codons richer in then the plant cells into which the gene is placed.___________________________________________________________ thymidine and adenine
33. Bacteria have also adapted to degrade man-made organics called .
34. Polychlorinated biphenyls (PCBs) are xenobiotics having high level of ….

Q35. Biological treatment are not best suited to use in which type of soil .?

36. Optimum PH for local environment conditions lies in the range of ….?______________________ 6.5–7.5
37. Which of the following treatment system is adopted for removal of biodegradable organic matter form domestic sewage? _______________________________________________ aerobic biofilm reactors
38. The sequence recognized by restriction enzyme is known as…_________________ recognition a site

Q39. High rate of ponds usually receive a high ….. load per unit surface area?

Q40. Which of the following method may be employed to improve the efficiency of pollutant degradation by a bacterium without the use of genetic engineering?

41. Which of the following technique is most suitable for the detection of RNA

Q42. Which of the following agents should be removed from tertiary level of water treatment level?

43. Microbes and enzymes typically function at __________________ lower temperatures and pressures.

Q44. Which of the following agent are used to support the formation of biofilm in low rate tricking filter?

Q45. Transgenic plants?

Q46. Which of the following chemical is removed from activated of sludge with biological nitrogen removal process?

47. The mechanism of separation of the particles with a density greater than sewage is adopted in …
48. The first bio tech based method of odor control was applied in..

Q49. Which gas is produced from up flow anaerobic sludge blanket reactor system?

50. The repeats flanking the T-DNA of agrobacterium tumefaciens are known as….

Q51. What is the main objective of maturation ponds?

52. In bacteria genetic transfer of material occur through …
53. Which of the following technique is most suitable for the detection of presence gene product.

Q54. A plasmid can be considered as a suitable cloning vector if?

Q55. which of the following is not characteristic of a transgenic crop?

56. What will be the sequences of application of waste water in wetlands in rainy season.

Q57. Main purpose of growing non food crop is to get …… commercially?

58. Which of the following group is now recognized as forming a distinct evolutionary line.
59. Restriction enzymes are enzymes

Q60. Which kind of filter is used in septic tank?

61. applications of the system overland flow is applicable to the …_________________________ plain area
62. bacillus thuringiensis tends to used codons richer in the plant cell into which the gene is placed.
63. generally the local environmental conditions required would ideally offer a temperature of.--- 20–30 ◦ C

Q64. Which of the following statement is false?

65. The existence of microbes capable of surviving in extreme environments has been known since the.. 1960s
66. mer B codes for which enzyme _______________________________________________________ lyase
67. a bacterial strain known to degrade PCBs is?_____________________________ Pseudomonas putida
68. lichens are used as bioindicators to monitor ……. Quality ____________________________________air
69. Extremozymes capable of functioning below pH1 have been isolated from these structures in some

…species __________________________________________________________________ acidophile

70. How many practical problems associated with GE of plants which make them more difficult to manipulate

than bacteria _________________________________________________________________________ 2

71. Of all soil which bacteria, seem to have the most highly developed ability to adapt quickly to new carbon sources
72. ….. are able to transfer genetic material between susceptible cells _______________________ eukaryotic viruses
73. Which this bacterium would later make possible the widespread use of a revolutionary technology, the polymerase

chain reaction (PCR) _________________________________________________________ Thermus aquaticus

74. A bacterial gene encoding pentaerythritol tetranitrate reductase, an enzyme involved in the degradation of

explosives, has been transferred into ______________________________________________ tobacco plant

75. biological treatments are not best suited to in which type of soils __________________________ all of the above
76. The oestrogen is degraded completely to …….. thus presenting a plausible method for water polishing prior to

consumption. __________________________________________________________ carbon dioxide and water

77. Of all soil bacteria …… seem to have the most highly developed ability to adapt quickly to new carbon sources.
78. Ralstonia eutropha, containing ……in its backbone, ____________________________________________ Sulphur
79. A use of thermophilic enzyme is seen in the application pfu polymerase for PCR . pfu stands for

________________________________________________________________ Pyrococcus furiosus

80. Reporter genes can only be a guide to the process of transcription and translation occurring in the cell

_________________________________________________________________________-_________ both b&c

81. Hyperthermophiles work at temperatures above _________________________________________85c
82. Many surface structural proteins in halophiles require severely elevated concentrations of ________.
83. Isolated samples of common place proteins, like egg albumin, are irreversibly denatured well at which

temperature? _______________________________________________________________blew 100c

84. Bryophytes are frequently used to monitor__________________________________. air contamination
85. The retention time of the solids in the system is called? ______________________________ sludge age
86. Planktons are often used as indicators of water pollution. Many planktons reproduce at an increased rate in

lakes when there are high centralizations of ____ and ___. ___________ phosphorus and nitrogen.

87. When the bacteria starts to use organic material from the cellular material of extended aeration system,

which kind of gas is produced ?

88. The removal of the organic matter in the secondary stage is carried out through biochemical reactions,

undertaken by.

89. Imhoff tanks, are basically____tanks? _________________________________________ sedimentation
90. Plankton also plays an important role in biological deterioration ___________________ organic matter
91. In bacteria, the most likely candidates for genetic transfer are_______. Bacteriophage
92. Bacteria have also adapted to degrade man-made organics called ______________________________xenobiotics.

Q93. Which of the following is not a characteristic of a transgenic crop?

Q94. What is the level of pH at which precipitation is done for removal of pollutants like phosphorus ?

95. Gene is expressed, preceded by a strong promoter, most commonly the ‘35S’ promoter, obtained

from_____________________________________________________. Agrobacterium tumefaciens