BIF401 Final Term Current Paper MCQs

BIF401 Current Paper MCQS

For finals

•  Genomics, evolutionary studies and system biology are application of bioinformatics
•  Exact matching require which type of nucleotide-same number of nucleotide
•  Accurate solution of protein structure is one of the ___ toughest problems
•  Tract back is overlap matches can start from any position in scoring matrix
•  The matrix has ………scores. Positive and negative
•  MASCOT___can search people mass finger printing and shotgun proteomics data set
•  Spectrometer measures the protein by _____ Mass/charge
•  Simulation of the folding process depends ______ Energy function
•  Information about DNA/RNA is available on gene bank
•  TDP is used to measure the molecules weight of intact proteins.
•  Coordinates of alpha carbon in the protein backbone can be used for structural visualization.
•  in structure visualization c-alpha atoms are traced to recreate a _____3D protein

structure

•  in UPGMA distance is calculated btw ______ two clusters and between two trees
•  pk value of Aspartic acid 3.9
•  Largest amino acid ………… Tryptophan
•  2nd STRUCTURE of proteins can be obtained by using_______ Chou Fasman algorithm
•  NJ Algorithm strategy is used to predict __ RNA 2` structure
•  The loop of hair pin must be at least ___ in length 2 aminoacids
•  Fold recognition is also called ……….threading
•  Ab initio method in contrast ,base their prediction on low energy model.
•  Bioinformatics require…….smart mind, and connected to internet, both,
•  Sequence alignment tool is…….PROSIGHT and MASCOT
•  MS1,MS2 provide us data identifying unknown…….Proteins sequence.
•  MGF files develop as an open standard for………. proteomics data.
•  PDB coordinates alpha carbon in protein back bone can be used for comparison .
•  Alpha carbon atom can be obtained from……PDB
•  in formation of 2nd structure of protein C & N can make……Hydrogen bonds
•  information for protein folding is into its native structure is in…….. protein’s amino acid

sequence!

•  Energy released during bond formation.
•  hydrogen bonding occur in …secondary ..Protein structure
•  Bond between C and H in alpha helices____Hydrogen bonding
•  Amino acid having 3 codons_____ isoleucine
•  MALDI typically adds a ………… to protein or peptide. Proton
•  low quality match gets_____lower score
•  ____ are sequences of amino acids produced during MS2. PST (peptide sequence tags)
•  MS2 data can be used to extract……… peptide sequence tags.
•  The alpha helices propensity should be more than____(1.0)
•  How many types are of protein sequencing techniques__ (2)
•  Blast can find sequences of _____nucleotides amino acid
•  If a protein sequence of 26 amino acids is fragmented at 11 amino acid C11, Z25
•  To find out unknown sequence of nucleotide we use . NGS or Mass spectrometry
•  How many types of peptide May be injected in mass spectrometry chamber.. . three

hundred thousand to four hundred thousand

•  .MASCOT.can search peptide mass finger printing and shotgun proteomics dataset.
•  Stabilizing and destabilizing energy give us …….. quality of 2nd structure .
•  The Fasman algorithm is based on statistical occurrence of Amino Acids in known structures.
•  DP recombinant the nucleotide recombination through process of ....... Traceback
•  MALDI.....add proton to a protein and a peptide..
•  Positively charged amino acids are..... 3
•  Aromatic amino acid include ....... . phenylalanine, tyrosine tryptophan
•  Homology modelling fail to predict ………… quality structure
•  How many forces are involved in protein folding. 4
•  Pairwise alignments tells the similarity between sequence... by maximizing the matches.
•  Dot plots employ dot matrix with two sequence plotted represents......pairwise alignment and comparison.
•  Differentiate b/w DNA and RNA sequence…………….. RNA has more variety of sequence
•  Cell molecule are produced after transformation of …………. DNA to protein
•  Dot plot cannot deal with…………. Insertion, deletion and gaps.
•  Exons are may be more ………… conserved.
•  In dynamic algorithm we can do comparison ………… of more sequence. Three
•  Mass/Charge ratio helps calculate the mass of the …. Protein
•  In scale tree branches lengths are equal to the magnitude of change in the …….. nodes.
•  How many types of peptides mix in MS chamber……….. 300,000 – 400,000
•  mRNA is a ………… structure. Planer
•  When unpaired bases of 2´ stricture join to form……….. 3´ structure
•  N Jackobson use to pridict 2´structure.
•  Aromatic amino acid ……. Tyrosine
•  Positive charge amino acids……. Lysine, arginine, histidin
•  MS begins with the measure of ………….. intact protein
•  Amount of amino acids in alpha helix…….(4)
•  Intact mass of protein can be found by….. MS1
•  MSA can be done by……. CLUSTAL
•  Which is used as sequence alignment tool……. PROSIT
•  MS1 and MS2 help to identify ……… (PST)
•  MGF is used for …….. proteomics
•  X,Y,Z positions of alpha carbon available online on …… (PDB)
•  Method to determine RNA structure …….. NMR, X-Ray crystallography
•  CLUSTLA runs………. Slow accurate/ fast appropriate
•  Which give info about precursor protein……. PSTS
•  Raw data files format can be converted to ………. Open format