BIF401 Final Term MCQs

bif401) (bioinformatics 1) MCQ’s for final term, Organised by Muhammad Kashif

151-250

------- provide mass of intact molecules and its fragments

MS1 and MS2

theoratical data is usually ----- as compared to exp. Data, larger Three scoring schemes can be applied to ------ score

the match at each stage of protein search

In the above scoring method, MW and PST stabs for ____ & _____ respectively

MS1 & MS2

_____ are assembled by Ribosomes in cellular cytoplasm

Proteins

Amino acids have characteristics like polarity, hydrophobicity, and charge states. these characteristics are governed by

Elemental composition of an amino acid’s side chain (R group)

Hydrophobic amino acids are non-polar due to very

little dipole moments between H and C

Core of amino acids is ______ and surface is ______ Hydrophobic

& hydrophilic

At pH ____ Five amino acids are charged, 2 negatively and 3 positively 7 pK values for an amino acid is the pH at which:

Exactly half of the chargeable group is charged

The pK of aspartic and glutamic acids are ___-& ____ respectively.

3.9 & 4.2

If pH < pK for an amino acid, the amine side chains____

Gain proton and hence basic

If pH > pK for an amino acid, _________ loses a proton and hence acidic

The carboxyl side chain

Lysine and arginine have pK ____ &___ respectively.

10.5 & 12.5

Glycine residues increase backbone flexibility because : They

have no R group (only an H)

______ reduce the flexibility of polypeptide chains.

Proline residues

____ Cement together by making _____ to stabilize 3-D protein structures

Cysteines, disulfide bonds

Chemically _____ remain in the core of protein.

Inactive amino acids

Backbone is highly polar (hydrophilic) due to polar ___ and ___ in each peptide unit

-NH & C=O

A-helices & B-sheets are stabilized by____ H-bonds The very few hydrophobic "patches" on protein surface are involved in:

Protein-protein interactions

Alpha Helix is Stabilized by H-bonds between every ____ in backbone

~ 4th residue

Proteins fold spontaneously to achieve:

Thermodynamic stability

If a protein is ____, then it can lead to a lack of function in the protein

Misfolded

Given algorithms and procedures to fold a protein, we can:

Fold amino acid chains to form 3D proteins

Each amino acid can fold into____, ____ & ____

Alpha Helices, Beta Sheets and Loops

If computations of protein folding take 1/10th of a nano-second(10 -10), then to compute all the folding possibilities will take

1.6 x 1030 years

All the information required for folding a protein into its native structure is present within the: