BIO203 MCQs Mega File by Biotech Brainy Bunch

1. ------------- An enzyme derived from the bacterium Thermus aquatics. (Taq

polymerase)

2. PCR can be performed to amplify--------- DNA
3. During extension the most common temperature -----------93C-98C
4. Which step the DNA polymerase synthesizes is new DNA strand complementary to DNA template strand by adding dNTP’s ? ---------- Extension
5. The thermo cycler works on the principle of -------------- which raise and lower the temperature of the block in a pre-programmed manner. Peliter effect
6. Primer are single stranded ------------- bp long DNA fragment 18—30
7. Which one of the following determines the specificity of PCR reaction? Primer
8. Formula for calculating melting temperature of primers. Tm = 4(G+C)

+2(A+T)

9. The extension rate of Taq polymerase ------------- 75nt/sec
10. Misprimers may occur due to nonspecific ------------of primer Hybridization
11. Control s for contamination contains all reagents except DNA template is called ------ --- Blank reaction.
12. Which type of PCR has very low probability of nonspecific amplification? NESTED

PCR

13. Two pair of PCR primer are used to amplify a fragment -------------NESTED PCR
14. ------------ IS a variant of PCR which enables simultaneous amplification of many targets of interest in one reaction by using more than one pair of primer.

Multiplex primer · BIO203 MID+FINAL M.C.Q.S MEGA FILE · GROUP: BIOTECH BRAINY BUNCH · ALL DATA FROM QUIZES + GRAND QUIZES · ADMINS: MEHWISH MUGHAL & BELLA

15. Which temperature for each of the primer sets must be optimized to work correctly within a single reaction. ANNEALING
16. Amplicon size should be different enough to form distinct bands when visualized by. ------ gel electrophoresis
17. The enzyme used to convert RNA into cDNA ------- Reverse transcriptase.
18. Which types of PCR is widely used in expression profiling, to determine the expression of gene? RT-PCR
19. ------------- Is used to measure the quantity of a PCR product. Real time PCR
20. Which is a technique that reduces nonspecific amplification during the initial set up stage of PCR? HOT START PCR
21. Allele specific PCR used for identify of ----------SNPs
22. In situ PCR is polymerase chain reaction that actually takes place inside the -----on a slide CELL
23. Flanking DNA sequences are digested and then ligated to generate ----------

Circular DNA

24. A method used to allow PCR when only one internal sequences is known--------

Inverse PCR

25. AFLP stands for ---------Amplified fragment length polymorphism
26. -----------is highly sensitive PCR-based method for detecting polymorphism in DNA.

AFLP

27. -------------Is a group of procedure that allow amplification to occur at many locations in genome. Whole genome amplification.
28. In mini primer PCR the thermostable enzyme extend primer consist -------nucleotides

9 or 10

29. PCR has been used in gene cloning and screening of ----------- Genomic

libraries

30. -------------is the application of scientific procedures to improve criminal and legal matters FORENSIC SCIENCE
31. -------------is a forensic technique used to identify individuals by characteristics of their DNA. DNA PROFILING OR DNA FINGERPRINTING
32. ------------- enzyme have symmetrical recognition sequences but are little value

Typelll

33. ------------- is used for the screening of bacterial or yeast clones for correct ligation or plasmid products. Colony PCR
34. Agaros extracted from sea weeds find used in ---------- Gel electrophoresis
35. ------------PCR uses the template for the reverse primer is a restriction fragment that has been self-ligated. Inverse
36. -------------tools have also allowed to perform prenatal genetic diagnosis.

Molecular

37. The instrument used to run the PCR is known as ---------- THERMOCYCLER
38. Non genomic material such as RNA can be detected by ------- In situ PCR
39. If the primer sequence is TCG, what section of DNA will it anneal? AGC
40. Which of the following is the source of pfu polymerase? Pyrococcus furiosus
41. -----------PCR uses primers whose 3’ ends encompass the SNP. Allele specific

PCR

42. M ethylation-specific PCR is used to identify patterns of DNA methylation at -----------

CpG island

43. At what temperature does annealing of DNA and primer takes place? 54C
44. For making cDNA --------- is used as a template RNA
45. A technique for making millions of copies of a specific region of DNA. POLYMERASE CHAIN REACTION.
46. The limitation of PCR are---------- all of these
47. Assembly PCR is used to assemble two or more pieces of DNA into -------- one piece
48. Binding of primer to single stranded DNA is called-------- Annealing
49. Two set of primer instead of one pair are uses in ----------- Nested PCR
50. Degenerate primer is used for making amplification of -------- Whole genome
51. PRIMER used for the process of polymerase chain reaction are ------------ (single

stranded DNA oligonucleotide)

52. Suicide PCR is used in ------- paleo genetics
53. Why are vent polymerase and Pfu more efficient than the Taq polymerase? (both)
54. A heat stable enzyme called Taq polymerase is used in polymerase chain reaction which is obtained from a bacterium ------------- Thermus aquatics
55. The process of reverse transcription reverse transcribes---------- RNA into DNA
56. ------------ is used as a template to produce DNA in Reverse Transcriptase PCR. RNA
57. ------------ may occur due to hybridization of primer to each other.

primer dimmers

58. ----------- determine the specificity of PCR reaction. Primer
59. ECOR1 is a ---------- restriction enzyme. Hexacutter
60. Primer can be designed ------------- to enable to initiate replication from a larger number of target locations. Degenerate
61. Which of the following is not a thermostable polymerase? DNA Polymerase lll
62. Sequence of nucleotides which have a twofold axis of rotational symmetry is known as ------------- Palindrome
63. A recognition sequence usually ranges from--------- bases. 4-8
64. Endonuclease are classified in to ----------main classes. 4