BIO302 Current Solved Papers

Q1. Crisprs Stands For?

Q2. Operon Model and Five Feature?

Groups of genes coding for related proteins are arranged in units known as operons. An operonconsists of an operator, promoter, regulator, and structural genes. The regulator gene codes for a repressor protein that binds to the operator, obstructing the promoter (thus, transcription) of the structural genes.

1. The products of the lacZ, lacY and lacA genes are encoded in a single polycistronic lac
2. The promoter for this mRNA molecule is immediately adjacent to the lac region.
3. The operator is a sequence of bases (in the DNA) to which the repressor protein binds.
4. When the repressor protein is bound to the operator, lac mRNA transcription can’t take place.
5. Inducers stimulate lac mRNA synthesis by binding to the repressor. This binding alters the

3. Name 2 Protiens Used In E.Coli

One, the lac repressor, acts as a lactose sensor. The other, catabolite activator protein (CAP), acts as a glucose sensor.

Q4. Torpedo Model?

An enzyme that degrades the second RNA as it emerges from the polymerase has been identified, and this enzyme may itself trigger termination. This is called the torpedo model of termination.

Q5. Involvement of CTD Tail In Transcription?

RNA polymerase II transcription elongation and Pol II CTD Ser2 phosphorylation: Atail of two kinases. Author information: ... The transition between initiation and productive elongation during RNA Polymerase II (Pol II) transcription is a well-appreciated point of regulation across many eukaryotes.

Q6. Two Protein Function And Name?

Lac sensor CAP catabolite

Q7. How Short life span of Mrna analysis Which Two Experimet Experiment Name (1) Experiment Explanation (2) ?

1st Expriment Name:

Pulse Chase

2nd Expreiment Pulse Chase:

Q8. How Many CTD Heptatidase In Repeat Sequence In Yeast Drosophila And Human?

There are 27 of these repeats in the yeast Pol II CTD, 45 in the fly Drosophila, and 52 in humans.

Q9. What ADAR? 3 Gene Close to Human ADAR?

Double-stranded RNA-specific adenosine deaminase is an enzyme that in humans is encoded by the ADAR gene (which stands for adenosine deaminase acting on RNA). ... The conversion from A to I in the RNA disrupt the normal A:U pairing which makes the RNA unstable. Humans and mice contain three ADAR genes: ADAR1, ADAR2, and ADAR3.

Q10. RNA Elongation In Three Lines?

elongation is the stage when the RNAstrand gets longer, thanks to the addition of new nucleotides. During elongation, RNA polymerase "walks" along one strand of DNA, known as the template strand, in the 3' to 5' direction.

Q11. How Regulatory Protein Control Gene Expression?

Regulated stages of gene expression. Any step of gene expression may be modulated, from the DNARNA transcription step to post-translational modification of a protein. The following is a list of stages where gene expression is regulated, the most extensively utilised point is Transcription

Q12. Write Point Operon?

an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter

Q13. Name Two Protein Which Is Present In E. Coli?

lac catabolite activator protein

Q14. RNA Polymerase 1 And 3 Function?

RNA polymerase I (RNAPI) transcribes rRNA genes, RNA polymerase II (RNAPII) transcribes mRNA, miRNA, snRNA, and snoRNA genes, and RNA polymerase III (RNAPIII) transcribes tRNA and 5S rRNA genes. This is in contrast with prokaryotes where a single RNA polymerase is responsible for the transcription of all genes.

Q15. Requriemt For Transcription?

important is the concept that transcription, whether prokaryotic or eukaryotic, has three main events. Initiation - binding of RNA polymerase to double-stranded DNA; this step involves a transition to single-strandedness in the region of binding; RNA polymerase binds at a sequence of DNA called the promoter.

Q16. Splicing?

splicing is the editing of a newly made precursor messenger RNA (pre-mRNA) transcript into a mature messenger RNA (mRNA). After splicing, introns are removed and exons are joined together.

Q17. Gene Expression?

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.

Q18. CRISPER and Application?

CRISPR technology is a simple yet powerful tool for editing genomes. It allows researchers to easily alter DNA sequences and modify gene function. Its many potential applications include correcting genetic defects, treating and preventing the spread of diseases and improving crops

Q19. Plus chase experiment, to verify life short span of mRNA?

Q20. Define Termination Sequence?

Once the polymerase has transcribed the length of the gene (or genes), it must stop and release the RNA product (as well as dissociating from the DNA itself). This step is called termination.

Q21. Write The Process Of Elongating Polymerase?

The Elongating Polymerase Is a processive machine that synthesizes and proofreads RNA. DNA passes through� the elongating enzyme in a manner very similar to its passage through the open complex. Thus, doublestranded DNA enters the front of the enzyme between the pincers. At the opening of the� catalytic cleft, the strands separate to follow different paths through the enzyme. The strands exit via their respective channels and re-form a double helix behind the elongating polymerase.

Q22. Five Steps Of Operon Model?

1. The products of the lacZ, lacY and lacA genes are encoded in a single polycistronic lac
2. The promoter for this mRNA molecule is immediately adjacent to the lac region.
3. The operator is a sequence of bases (in the DNA) to which the repressor protein binds.
4. When the repressor protein is bound to the operator, lac mRNA transcription can’t take place.
5. Inducers stimulate lac mRNA synthesis by binding to the repressor. This binding alters the