BIO302 MCQs Mega File by Biotech Brainy Bunch

1. An E. coli chromosome is estimated to have about_____ super-coiled loops. (400)
2. The first step is the initial binding of polymerase to a promoter to form what is called____? (Closed complex)
3. tRNA accounts for some____ of all cellular RNA. (10%)
4. Polycyclic aromatic hydrocarbons (PAHs) formed by incomplete combustion of the burning wood or____ (Coal)
5. The____ model predicts that the linker DNA forms a straight path between nucleosomes____. (Zigzag)
6. tRNA having amino acid joins with____ during protein synthesis. (mRNA)
7. Hershey and chase used____ isotopes for their experiments. (P)
8. In a molecule of double-stranded DNA, the amount of Adenine present equal to the amount of? (thymine)
9. Cell free fermentation was discovered in_____ (1897)
10. The wave length range of VU-B light is____ nm. (296- 320)
11. Direct de-alkylation reactions have probably been most extensively studied in_____. (E.

coli)

12. The bacterium used in Hershey and Chase experiments were____. (E. coli)
13. The nucleosome of eukaryotic genome contains about____ protein molecules with DNA.

(9)

14. The wavelength of ultra violet light that damages DNA is_____ nm. (100- 400)
15. Which three additional enzyme are required when the nick is present on 3’ end the mismatch. (MutS-alpha, Exol, and RPA)
16. Presence of 5 origins of replication will divide each chromosome into_____ replicons. (5)
17. RNA polymerase functions only in the presence of_____. (DNA)

BIO302 MID+FINAL M.C.Q.S MEGA FILE · GROUP: BIOTECH BRAINY BUNCH · ALL DATA FROM QUIZES + GRAND QUIZES · ADMINS: MEHWISH MUGHAL & BELLA

18. ______ number of distinct DNA glycosylase/ lyase enzymes have been identified in human cells. (6)
19. Which shows the correct complimentary base pairing of DNA? (T-A, G-C)
20. Long patch repair pathway replaces the_____ nucleotides. (2- 8)
21. Ribose and deoxyribose sugar in nucleic acids differ in___ at___. (N, C3)
22. E. coli chromosome consists of approx.____ nucleotide pairs. (4 Million)
23. All organisms that have a mismatch repair system have MutS and _____ homologs.

(MutL)

24. Formation of cyclo-butane pyrimidine dimer (CPD) which accounts for about ____ % of all the UV_ induced damage. (75)
25. DNA replication is semi- conservative was proved in____. (1958)
26. Nucleoside is_____. (Nucleotide- Phosphoric acid)
27. The bond angles in protein primary structure resulting from rotations at C are labelled (phi) for the____. (N- alpha C)
28. Cisplatin is very effective chemotherapeutic agent for treating cancers of the____.

(Bladder, Testicles, Ovaries, All given)

29. The first enzyme diastase was discovered by_____. (Payen)
30. Key substrates required for the synthesis of DNA to proceeds are_____ and____.

(Deoxy-nucleoside trio-phosphate- primer)

31. DNA polymerases introduce about one miss-paired nucleotide per____ nucleotides.

(105)

32. Performed the X-ray diffraction analysis of DNA fibers. (Rosalind and Wilkins)
33. Exole enzyme degrades DNA in a_____ direction. (3’…….5’)
34. _____carries the information from DNA to the ribosomal sites of protein synthesis.

(mRNA)

35. Symmetry in quaternary structure of protein with two fold rotational axis is called____.

(Dihedral)

36. RNA creates cavities on their outer surfaces specific for the different_____. (Amino

Acids)

37. _____ make RNA using appropriate segments of chromosomal DNA as their templates.

(RNA polymerase)

38. The first discovered enzyme is known as____. (Diastase)
39. _____ adds a deoxy-ribonucleotide to the 3’-OH and of DNA. (DNA polymerase)
40. The movement of DNA helicae along single strand of DNA strand in defined direction is known as____. (Polarity)
41. An AP (apurinic and apyrimidine) site can be produced by___ enzyme. (DNA

glycosylase)

42. Meselson and Stahl performed a classical experiment to distinguish among three possible models of DNA replication using isotope of____. (N)
43. _____ is a sulphur containing amino acid. (Methionine)
44. CH3 group is attached at____ of thymine nitrogenous base. (C6)
45. Psoralen can_____ DNA if photo-activated. (Alkylate)
46. Which one of these amino acids are present in high percentage in histone protein.

(Lycine)

47. One of the most mutagenic base analogs is 5-bromouracil, an analog of_____. (Thymine)
48. The basic nucleotide excision repair pathway is____ in all the organism. (Same)
49. The length of mRNA molecules is variable and depends on the length of____. (Protein)
50. James Watson and Francis Crick discovered the double helical structures of DNA in___.

(1953)

51. Which one of these amino acids are present in high percentage in histone protein.

(Arginine)

52. Central dogma of molecular biology does not involve____. (Carbohydrates)
53. CPD photolyases are present in all organisms except___. (Humans)
54. Urea was synthesized Wohler in____. (1828)
55. The incorporation rate of rNTPs is____ folds lower than dNTPs. (1000)
56. Which of the following is not a base of RNA? (Thymine(T)

Q57. Which enzyme prevents the accumulation of supercoils ahead of the replication fork?

(helicase)

58. Working independently cracked the code in the early 1960s. (Nirenberrg and Gobind)
59. The hydrophilic backbones of alternating deoxyribose and phosphate groups are inside the double helix, facing the surrounding water? (False)
60. Chromosomes are made up of? (Protein and DNA)
61. Chromosome theory of inheritance by Thomas Hunt Morgan was presented in? (1910 )
62. Nucleotide consists of …… major molecules. (5)
63. How many amino acids are there in one complete tern of DNA? ( 3.6 )
64. Nucleosomes fold to form the _______ fiber. ( 30 nm )
65. On which media, cells are preferred to be grown? (N14 b)
66. DNA polymerase has ________ domains. (3 )
67. An E.Coli chromosome is estimated to have about............super-coiled loops. (400)
68. Separation of DNA is accomplished by __________. (Type II topoisomerase)
69. Which one of the following is not an ionizing radiation? (Ultraviolet light)
70. Hydrolytic deamination of cytosine is estimated to take place about a…. ( 100 to 500

times a day in a mammalian cell)

71. What will happen to the DNA exposed to N-methylguanine. ( it will form a basic site )
72. Aflatoxins are produced by; ( Fungi)
73. ............are similar enough to the proper bases to get taken up by cells converted into nucleoside triphosphate and incorporated into DNA during replication. ( Base analogs)
74. Cells infected with phage ..........provide the ideal system to find the true template for protein synthesis ( T4 )