BT102 Midterm Past Papers by Dining Discussion

OBJECTIVES

1. After gram staining, gram positive bacteria appear: Purple (crystal violet)
2. Slide or specimen is placed on…………….Stage Of Microscope
3. Kingdom Monera consist of---------- Archea + Bacteria
4. Which is used for mordant in gram staining?......................Iodine
5. The primary stain is used in acid fast staining is …………….Carbol Fuchsin.
6. Aristotle thought that Simpler invertebrate could arise by spontaneous generation.
7. Light microscope power……………….0.2
8. Robert Hooke First drawing of microbes in …………….1665.
9. Which One is the basic dye-------------Methylene Blue
10. Study Of Microbes………………Microbiology
11. Mycobacterium is stained by…………..Acid-Fast Technique.
12. Microbes also called germs or microorganisms are … to be seen with unaided eye.

Too small

13. … used to be the main feature prior to the advent of molecular biology.

Morphology

14. Bacteria and archea are two … groups. Different
15. … are composed of only circular ssRNA. Viroids
16. … are also called satellite viruses. Virusoids
17. … are infectious proteins. Prions
18. Linnaeus in … established system of specific nomenclature. 1735
19. Each organism has two names, write: Genus & specific epithet
20. Genus is … and epithet is Capitalized, lowercase
21. … is found in large intestine & … is on skin. E. Coli, S. aureus
22. In the presence of … bacteria grow and change alcohol to acetic acid.--- O2
23. In 1860 Joseph Lister applied … as treating surgical wounds & hand sanitizing.

Phenol

24. Isolation of bacteria made possible by … and …. Agar & Petri Dish
25. Archea and bacteria differ in rRNA
26. The period from 1857 to 1914 was rightly called as the Golden Age Of

Microbiology

27. Use of microbes or their enzymes for large scale production of biomolecules:

Industrial microbiology

28. Using a … loop, a colony or a drop of broth culture can be smeared into a thin film on a glass slide for making a smear. Platinum
29. While drying the smear … can be used. Methyl alcohol
30. A colored ion: Chromophore
31. The color is in negative ion: Acidic dye
32. Most commonly used for bacteria: Basic dyes
33. Only one stain is used ---------- Simple staining
34. A couple of stains is used: --------- Differential staining
35. Oil immersion power of objective lens 100x
36. Low magnification power of ocular lens: 100x
37. Ability of the lenses to distinguish between two closely lying objects as separate:

Resolution

38. Kingdom Monera consist of: Archea + Bacteria
39. Which of the following contains mycolic acid?---- Mycobacterium & Nocordia
40. Rod shaped bacteria are called: ------ Bacilli
41. Energy is required in: -------- Active transport
42. Which of the following structure is involved in the locomotion of bacteria? ----

Flagella

43. Gram positive bacteria produced: --------- Exotoxins
44. The basal body of flagellum of gram-positive bacteria consist of … rings. -2
45. 70s ribosome consist of two subunits: --------- 50s & 30s
46. … are used for twitching motility. ------- Pili
47. Integral proteins are … in the cell membrane and are … in water. ------Imbedded,

Insoluble

48. Which of the following is not present in bacteria? ------ Both Histone & Nuclear

Membrane

49. Specialized resting cells: -------- Endospores
50. Movement against the concentration gradient: -------- Active movement
51. Cytoplasm contains … percent water. -------- 80%
52. Which of the following condition in which water moves into the cell? --------

Hypotonic

53. Which of the following initiate the spore activation?---------- Heating
54. Facilitated diffusion is done with the help of: -------Integral Proteins
55. Which is used to transform DNA from bacteria to another? -----------Pili
56. … is also called volutin. ------- Metachromatic Granules
57. Peripheral proteins are: Loosely Connected To Plasma Membrane
58. Magnetosomes are: Inclusions Of Iron Oxide
59. Gram negative bacteria has … rings: ----------- 4
60. Nucleoid & ribosomes are formed: ----------- Spore Core
61. … gives structural support. ----------- LPS, Core Polysaccharide
62. Rat bite fever is caused by: -------- Spirillum Minus
63. Bacterial cell wall is made up of: ---------- Peptidoglycan
64. … are circular or spherical in shape. ------------ Coccus
65. A resistant dormant structure within a cell? -------- Endospore
66. Some bacteria such as … have a waxy material in their cell wall. --------

Mycobacteria

67. Spirochete are: ---------- Spiral
68. When a cell is neither bacillus nor coccus and it is intermediate b/w two extremes:- --------Coccobacillus
69. … distributed over the entire cell. ---------- Peritrichous
70. … is a viscous & gelatinous secretion that surrounds the cells.----------

Glycocalyx

71. If glycocalyx is organized & firmly attached to the cell. ----------- Capsule
72. Axial filaments are also called: ------ Endo-Flagella
73. Axial filaments are only present in:------- Spirochete
74. … help bacteria to attach to surface before they can secrete biofilm.------

Fimbriae

75. … are hair like structures composed of pilin: --------- Fimbriae