BIO203 Quiz 1 by Team VU Legends

Bio203 QUIZ NO 1 · FROM LACTURE (15 TO 25) · Effort by team of Vu Legends

1. Long PCR is usful only when it is ................ Accurate
2. Extended or longer than ........... PCR meaning over ........... kilobases Standard , 10
3. Special mixture of proficient polymerases along with accurate polymerases such as ................are often mixed together. pfu
4. Application of clone large gene is not possible with .................. Conventional PCR
5. Allele- specific PCR used for identifying of ............. SNPs
6. ................requires prior knowledge of a DNA sequence, including difference between alleles. Allele-specific PCR
7. Allele-specific PCR uses primers whoes .......... ends encompass the SNP. 3' ends
8. PCR amplification under the stringent condition is ............... in the presencde of mismatch between tmplate and primer. Much less efficient
9. Successful amplification with an ............... signals presence of the specific SNP in a sequence. SNP- specific primer signals

Q10. Which technique is used to identify or utilize sigal-nucleotide polymorphisims (SNPs)?

Diagnostic or Cloning technique

11. ............. is the screening of bacterial or yeat clones for correct ligation or plasmid products. Colony PCR
12. Heat the mixture of colony PCR in a boiling water bath for ........... minutes. 2 minutes
13. Spin sample for 2 minutes high speed in a ........... Centrifuge
14. .................. is a polymerase chain reaction that actaully take place inside the celle on a slide. In situ PCR
15. In situ PCR amplification can be performed on ............ Fixed tissue and cells
16. In situ PCR allows the identification of ........... Cellular marker
17. RNA is a ............ Non genomic material
18. In situ PCR is limited to detection of ............... Non genomic material
19. ............. uses Standard PCR primer oriented in the reverse direction of the usual orientation. Inverse PCR
20. The tamplate of the reverse primer is a ............. that has been self ligated. Restriction fragment
21. Inverse PCR funtion to ................. a known sequence. Clone sqeuences flanking
22. ............ are digested and then ligated to generate circular DNA. Flanking DNA sequences
23. ............... is a method used to allow PCR when only one internaol sequence is known.

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