BT301 MCQs Mega File by Biotech Brainy Bunch

Midterm Portion

1Adjacent nucleotides are joined together by ------. (Phosphodiester bond)

5- -------- give the most important clue of structure of DNA before 1953? (Erwin Chargaff) 6A famous specific book written by Robert Hooke that brought microscopy into scientific limelight was -------. (Micrographia) 7The sequences within a gene that encode proteins sequence are called -----. (Exons) 8The most common secondary structure is --------. (Alpha helix) 9What is incorrect about plasmids? (Serves as the transformation vehicles) 10In 1878, isolated non protein component of nucleon and discovered nucleic acids and isolated 5 bases: A, T, G, C and U. (Albrecht Kossel) 11The recognition sequence of the Haelll restriction enzyme is ------. (GGCC) 12We sequence the Genome to-------. (Identify genes, gene location and gene regulation, All of the above) 13In human about ------ genes while in bacteria about-------. (23000 & 2000)

15Enzymes that remove nucleotides one at a time from the end of a DNA molecule are called--- ------. (Exonucleases) 16The DNA modifying enzyme capable of making DNA complementary to RNA is? (DNA

polymerase) · BT301 MID+FINAL M.C.Q.S MEGA FILE · GROUP: BIOTECH BRAINY BUNCH · ALL DATA FROM QUIZES + GRAND QUIZES · ADMINS: MEHWISH MUGHAL & BELLA

17Terminal deoxynucleotidyltransferase s an enzyme purified from ------- used in homopolymer tailing? (Calf thymus) 18Liposomes are the vesicles that are made up of --------. (Phospholipids) 19The process by which DNA gel is transferred to a membrane is termed as ------.

(Electrophoresis)

20Bacteria are small organisms carry high coding density than ------. (Animals and plants) 21Sumerians and Babylonians used anaerobic respiration of yeast to make beer in ------. (6000

BC)

22In autoradiography we use ------ film to visualize the radioisotope. (X-rays)

26The technique in which DNA is labeled using complementary sequences and then analyzed using audio radiograph is called------- (Southern blotting) 27Runaway plasmids don’t have control to --------- (copy number) 28Which of the following is a mismatch? (Templatedouble stranded DNA) 29In his famous transformation experiment in 1928. Frederick Griffith performed experiments on ------- strains. (Streptococcus pneumoniae) 30The percentage of nucleic acid in relation to the protein is about----- for influenza virus.

(1%)

31An enzyme known as ------introduces nicks into super-coiled loops of DNA. (DNase) 32The ----- have blunt ended DNA molecules while ----- have cohesive ends. (Adaptors,

linkers)

33Amino acids have ------- (Both amino group and carboxyl group) 34Which one of the following represents a palindromic sequence in DNA? (GTTCCAAG) 35The agent used to denature proteins and give the negative charge during western blotting is known as ----- (Sodium Dodecyl sulfate) 36The enzyme used to digest many containing proteins during the isolation of genomic DNA from E. coli is called --------- (Proteinase K) 37The genome of polio virus is about ----- nucleotides long. (7500) 38LINEs possess ----- ORFs which encode all the functions needed for retro transposition.

(two)

39The genome size of ----- is 120kb. (pisum sativum) 40Which of the following is not the component of DNA? (Thymine) 41- ------- is the enzyme which helps to prevent recirculation of DNA. (Alkaline

phosphatase)

42The repeating units of proteins are-----. (amino acids) 43If copy number is low then plasmids are called as -----. (stringent plasmids) 44First system to be characterized from E. coli k12 was? (type 1) 45The temperature required for the denaturation of DNA double helix is -----. (94) 46Linkers have? (one end sticky and other end is blunt) 47Nick produced by DNA strand is repaired by---- (DNA ligase) 48T4 DNA ligase works more efficiently to join fragments of ---- ends? (Blunt ends) 49The---- backbones of alternating deoxyribose and phosphate groups are outside the double helix facing the surrounding water. (hydrophilic) 50One circle or OC DNA has ----- stranded intact. (One) 51Among the following, the electrophoresis cannot be used for them ------. (Separation of

lipids)

52The SINE commonly contains the restriction sites for the endonuclease------. (Alu 1) 53Genomics is the study of genomes. Genome refers to the ----. (entire set of genes of an

organism)

54The change In the frequency of a gene variant in a population due to random sampling of organisms is called-----. (genetic drift) 55The stop codon is-----. (UAA) 56The use of salt for preserving food especially meal was started in ------. (400 BC) 57The RNA molecule which takes part in the formation of ribosomes is------. (rRNA) 58Father of genetics was the ----- (Mendel) 59The substance which causes of the precipitation of DNA is------. (Ethanol) 60Circular DNA molecule that replicates separately from the host chromosome are ----.

(Plasmid)

61The most commonly used enzyme for joining the blunt and DNA molecules. (T4 DNA

ligase)

62The size of the human genome is -----. (3000 MB) 63The branch of Biotechnology that embraces all methods of genetic modification by recombinant DNA and cell fusion techniques is called --------. (Modern biotechnology) 64Which of the following can be used as a vector for cloning? (Plasmids, YACs and BACs) 65The system which recognizes a particular target sequence and does not require co factor is --- --. (Type 11) 66The process of copying of mobile DNA element and insertion into new site in genome-------.

(Transposition)

67Messenger RA is always -------- stranded when formed from DNA. (single) 68Large polypeptides chains usually fold into two or ore globular clusters known as ------.

(domains)

69The electrophoresis apparatus consists of ------. (electrophoresis unit and gel separator) 70Those plasmids to which phenotypic traits have not been ascribed are called-----. (Cryptic

plasmids)

71Which of the following terms refers to the order in which amino acids are linked together in a protein? (primary) 72The function of messenger RNA is that--------. (it is a direct copy of gene) 73All of the following are considered weak interactions I proteins except ------. (ionic bond)