BT301 Quiz 2

BT301 - Introduction to Biotechnology

QUIZ NO. 2

1. Phasmid vectors are combination of plasmid and ʎ phage sequences .
2. A great advantage of hybridization for library screening is that it is extremely

versatile .

3. (YACs) are the Yeast artificial chromosomes used for large DNA fragments insertions.
4. Vectors that can replicate & are stably maintained in two or more unrelated host organisms are called shuttle vectors.
5. For cDNA expression libraries the correct orientation achieved by self-priming method by adding linker molecule to double stranded cDNA .
6. Cosmid are the Plasmids containing a fragment of ʎ DNA including ‘cos’ site .
7. Which statement is correct: Bacterial artificial chromosomes for they are simply plasmids designed for the cloning of very long segments of DNA .
8. cDNA library is representative of the RNA population from which it was derived host.
9. In Genomic DNA libraries in appropriately sized random fragments be produced restriction endonucleases.
10. PCR with specific primers could be used to isolate genes directly from genomic DNA.
11. Major screening strategies involve all of these.
12. BamH1 enzyme used in a Creation of genomic DNA library using the phage-ʎ vector EMBL3A
13. Benton and Davis (1977) devised a method called plaque lift.
14. Bacterial artificial chromosomes are simply plasmids designed for the cloning of very long segments of DNA .
15. Synthesis od cDNA from RNA is done by using RT-PCR.
16. Vectors that can replicate & are stably maintained in two or more unrelated host organisms are called shuttle vectors.
17. Genomic DNA libraries constructed using high-capacity vectors .
18. cDNA is sequence that lack introns sequence.
19. M13 is a filamentous bacteriophage with single stranded DNA that replicates without killing its host .