MB504T Midterm MCQs 2024 by Miss Mehwish

MB504T (Mega File) · Mid Term (Live Quiz) · Past Papers for Mids./Finals are also Available

The type of PCR used for the screening of bacterial or yeast clones for correct ligation or plasmid products is _______. Insitu PCR Mini primer PCR Multiplex PCR

2. _ can be also used for genotyping individuals for a large number of loci. Insitu PCR RFLP AFLP ✔️ ARMS-PCR

Q3. Synthesis of cDNA takes place using oligo dT primers at what conditions?

40°C two hours 25°C one hour

37°C one hour ✔️

18°C one hour

Q4. Which type of restriction enzyme is EcoRI?

Hexacutter ✔️

Pentacutter Polycutter Tetracutter

5. PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated. Inverse ✔️ Allele-specific Colony Asymmetric

6. RT-PCR is widely used in:

Expression profiling ✔️

RNA editing Protein expression DNA amplification

7. EcoRI is a ______ enzyme that can digest the genomic DNA. Polycutter

Hexacutter ✔️

Penta cutter Tetra cutter

8. PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products. Allele-specific PCR

QPCR

9. Allele-specific PCR uses primers whose ___ encompass the SNP. Promoter region 2' ends 3' ends ✔️ Sends
10. _ is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. Conventional PCR ARMS PCR

Which of the following statements is accurate for the PCR?

Millions to billions of desired DNA copies can be produced from microgram quantities of DNA A thermostable DNA polymerase is required Automated PCR machines are called thermal cyclers

 Which of the following is the first and the most important step in the polymerase chain reaction?

Denaturation ✔️

Annealing Final extension Primer extension

 How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?

16 ✔️