BIO203 Final Term Current Paper Solution 2023 by Muhammad Zaman

Mcqs:

(Mostly from Files)

•  PCR _________ Polymerase chain reaction
•  RFLP________ Restriction fragment length polymorphism
•  Random Amplified Polymorphic DNA_____RAPD
•  Amplified fragment length polymorphism_________AFLP
•  which is type of microscope having beams of electrons__________ Electron Microscope
•  Which type of pcr is done inside the cell__________In

Situ PCR

•  The type of pcr which defines bacterial yeast clones is.......... Colony PCR
•  STR stands for......Short Tandem Repeats
•  How many Screening Methods SNPs have.......2
•  WGA PCR stands for....... Whole Genome

Amplification Polymerase Chain Reaction

•  To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________.Asymmetric Pcr
•  Type of pcr which assembel many pieces into one..........

Assembly Pcr

•  Immunosorbent Electron Microscopy contains............ antibodies. Both (monoclonal and polyclonal)
•  In Gel Electrophoresis molecules movement depend on ......... Size
•  10% DMSO Works well as a........Freezing Medium
•  Antibodies recognize a unique molecule called........

Antigen

•  How many % attribute make a men unique. 0.1%
•  Multiplex-PCR consists of multiple primer sets within a ------Single------ PCR mixture to produce amplicon of varying sizes that are specific to different DNA sequences.
•  The PCR is preceded by a reaction using reverse transcriptase to convert ----------------- RNA to cDNA
•  Real Time PCR is a technique in which --------- fluorophores --------- bind to specific target regions of amplicons to produce fluorescence during PCR.
•  Allele-specific PCR used for identifying of -------- ------ SNPs.
•  Colony PCR_ The screening of bacterial or yeast clones for correct ligation or plasmid products.
•  IN SITU PCR is a polymerase chain reaction that actually takes place inside the cell on a slide