BIO203 Final Term Past Papers Spring 2021 by Mehwish Mughal

MCQs from PPTs & Past Papers

Q1. Restrictions enzyme type II and type IIs (2 marks)

Q2. Principles family of (VNTRs) (2 marks)

Q3. Write the Full name of RFLP ,AFLP, RAPD (3 marks)

Q4. Nomenclature explain (3 marks)

Q5. Four restrictions and modification system (5 marks)

6. Haplotype corelate phenotype

Q7. How many technique detect polymorphism (5 marks)

Q7. How many technique detect polymorphism 5 marks 7.How many technique detect polymorphism 5 marks 8. TypeII and type IIs ma diiference (2 marks)

•  name of the support solid component and hybirdized component of the in southern blotting 2 markx
•  nomenclature for restriction enzyme 3 markx
•  electrophrosis ka principle 3markx
•  3no blotting mai difference 5 markx
•  name of transfer method in explain upward method 5 mrkx

Q9. R-M system (5 marks)

10. DNA precipitation and steps of purification (5marks) 11. Enzymes involved in DNA modification (2marks) 12. Colony PCR (2marks) 13. SNP maps (3marks) 14. Mechanism of Ligase activity (3marks) 15. Colony PCR, 16. SNP. 17. Restriction Modification System. 18. DNA fingerprinting. 19. Applications of DNA fingerprinting. 20. Name solid support and hybridizing components to immobilize dna in southern blotting 21. Applications of pcr in clinical microbiology 22. How haplotype correlate with phenotype 5 marks 23. Write a note on restriction modification systems. 5 marks 24. Which features of radioactive probe makes it use for southern blotting 25. Write different techniques to detect unknown polymorphism. 26. .enlist method for DNA transfer to membranes. And moreover, procedure for upward transfer of DNA (5) 27. How does haplotype correlates with phenotype (5) 28. Applications of Western blotting (3) 29. Methods for DNA transfer (3) 30. Southern blotting (2) 31. Why secondary antibody is benefit for Western blotting (2)