BT301 Final Term Current Papers Spring 2024 Set 2 by VUBuddy

•  Erwin Chargaff work in... (1940)
•  One of the applications of chromosome walking and jumping is in the cloning of humans-----gene (Cystic fibrosis)
•  Technique used to identify individuals by characteristics of their DNA sequences is called----(DNA profiling and fingerprinting)
•  LTR consist of ------ bp (250-600bp)
•  Somatostatin protein is prevented from degradation by------ (Beta

galactosidase)

•  cDNA lacks the --------- sequences which are present in genomic

DNA. (intrinsic)

•  Mitochondrial gene in human cell... 37
•  Techniques for DNA sequencing became available in the... Late1970s
•  ---------is a type of PCR reaction but the segments of DNA that are amplified are random (RAPD)
•  Sequences that were read beyond 4oo bp contained an average of ____3.2%
•  The molecular weight of pBR322 vector is ----- (4361 bp)
•  In case of mammals or fungi, the genome size of mitochondria ranges from 15- ----- kb. (20)
•  Real time PCR also known as (Q- PCR)

promoter)

•  Complementarity of Shine-Dalgarno (S-D) sequence with 16s rRNA can affect the... Rate of translation
•  When the R groups are arranged in a beta pleated sheets. (outer side)
•  Happy mapping in genome mapping is an entirely------- technique. (in vitro)
•  When distances in genome mapping are calculated in terms of units then this map is known------ (Physical map)
•  The rate of synthesis of a particular protein will depend on the steady- rate of---- in the cell. (mRNA)
•  The genome size of the nanoarchaeum equitant is ----- (0.49)
•  A type of PCR reaction in which segments of DNA that are amplified are random is -------- (RAPD)
•  Particular transcript of mRNA in a cell is degraded by ------- to lower the expression of particular gene. (mRNA)
•  Mitochondria generate in human cell (13)
•  A shutter vector is____ (A vector that can be used with two/more systems)
•  Sequences that are read beyond 400 bp contains an average of (3.2%)
•  A term used to describe an approach which allows the isolation of gene sequences which is known as------ (chromosome walking)
•  Phage display is the technology that allows the expression of ----- on the surface of phage particles. (Exogenous polypeptides)
•  A modified procedure for cosmid is derived by Ish-Horowicz and

Burke

•  genome size of human 2.8 x 106
•  In --------two sets of primers are used in two successive PCRs

(Nested PCR)

•  Kuhne creates the term “enzymes” and discovered.......( trypsin. )
•  Boyer brought in restriction enzymes ...(1973)
•  The overall three-dimensional arrangement of all atoms in a protein is referred to as the protein’s ....(tertiary structure)
•  mans have genome size of (3,000 Mb)