BT301 Final Term Objective

in pBR322 replicon element is pBR11

pMB1

RSf2124 PstI pBR322 have -----target sites for different restriction enzymes

pBR322 have high copy number and -----weight High

Low

pBR325 encodes ----resistance in addition to those resistance have psc101 plasmid.

Chloramphenicol

pAT153 another derivative of pBR325

pBR322

psc101 plasmids which loss during cell division is called High copy number plasmid Low copy number plasmid

Runaway plasmid

Lambda phage vector Wild type is not suitable as a cloning vector because it has ----restriction enzymes sites Very low number of

Too many

A few Charon4A is a replacement vector is this statement

True

False Charon 60 is a replacement vector is this statement True

False

Charon 60 has ----- restriction site

Many Charon 4A and 16 are both derivatives of ------ Bacteriophage

Lambda

Ecoli Charon 60 can be cloned 2 kbp 3kbp 4kbp

5kbp

lacZ gene encode for enzyme PAL 4CA

βGal

PPR first step in the cloning is Cutting vector

Isolation of DNA

Insertion of vector Infection in case phage-λ Packaging yields about ----- of vector DNA 104 plaques/ µg 105 plaques/ µg

106 plaques/ µg

At each cos site nicks are introduce ----- far apart on opposite strand of DNA 10bp 13bp

12bp

16bp Cos sits are Cloning site Cooperative site

Cohesive site

Capsulation site Gene D is include in the --- process

Capsulation

Ligation Termination Packaging M13 is a filamentous bacteriophage with----- dsDNA

ssDNA

dsRNA ssRNA Which of the following vector replicate without killing its host Phage-λ

M13

Mp18 Cosmid M13 can clone DNA of variable length about 3kpb 4kbp

5kbp

6kbp In x gal containing medium blue colonies represent the cloned DNA colonies, statement is True

False

Cosmid can clone around ------length of DNA

45kbp

37kbp 53kbp 200kbp Modified cosmid vector produced by ----- in 1981 Watson and crick

A modified procedure for cosmid is derived by Watson and crick

Cosmid c2XB is derived by Watson and crick

-----vectors are combination of plasmid and ʎ phage sequences Lambda

Phasmid

Cosmid BACs can clone ----------length of DNA fragment 5kbp 150-200kbp

100-300 kbp

60kbp YACs vectors replicate in ------like normal chromosome Bacteria

Yeast

Fungi Mammalian cell YACs can clone ----------length of DNA fragment

5kbp

150-200kbp

100-300 kbp