BT301 Final Term Past Papers by Asmat Khan Naizi

Bt-301

1st ExcEllEnt Zoologist

1Structual instability of plasmids may arise by deletion or arrangements of ……….

•  DNA

2Major screening strategies involve ………….

•  Genetic methods
•  Sequence dependent screenings
•  Screening expression libraries
•  All of these

3- Functional complementation also possible in …

•  Transgenic animals and plants

4Gram-negative bacteria such as E. coli have a ….

•  Complex wall membrane

5In chromosome jumping the DNA of interest is identified cut into fragments with restriction enzymes and …………..

•  Circularized

6- …………………. Depends upon transcription of appropriate gene, efficient translation of mRNA and in many cases, post translational processing and compartmentalization of nascent

•  Synthesis of functional protein

7Immunochmical screening involves the use of ……..

•  Antibodies

8If DNA liberary is established using expression vectors each individual clone can be expressed to yield a …….

9One way of increasing the expression of a clone gene is to increase the number of the ………..

•  Plasmid copy number

10Complementarity of Shine Dalagamo (S-D) sequence with 16s r RNA can affect the …..

•  Rate of translation

11This is the process by which a particular DNA sequence compensates for a missing function in a mutant cell and thus restores the wild type phenotype.

•  Functional complementation

12- Synthesis of c DNA from RNA is done using …

•  RT+ PCR

13To isolate specific clone PCR is carried out with gene specific primers.

•  Gene specific primers

14Expression from a strong promoter can represent ……… of cloned gene product of total cell protein.

•  20-40%

15Benton and Davis (1977) devised a method called.

•  Plaque lift

16- pBR322 is an example of in

constructed cloning vehicle.

•  Intro
•  Extro

 Vitro

•  In-extro

17-

It is difficult to clone DNA segments longer than about

when plasmids are used as the vector

•  10,000 bp

 15,000 bp

•  20,000 bp

25,000 bp

1. pBS322

20- pUC19 carrying sites formany different

endonucleases

a. addition b. active c. moving

d. restriction

21Charon 4A and 16 Each has the lacZ gene, encoding enzyme βgalactosidase .

22- Connecting the two lambda fragments to foreign DNAby using

a. DNA ligase

b. Polymarise c. RNA d. Polymarise II

23- Packaging in vitro yields about

of vector DNA

a. 104 plaques/ µg b. 105 plaques/ µg

c. 106 plaques/ µg

d. 107 plaques/ µg

24-Plasmids have been constructed which contain a fragment of ʎ DNA including

a. Sin site

b. cos site

c. tan site

25Cosmids are particularly attractive vectors for constructinglibraries of fragments .