ZOO101 Merged PPT Lectures 1-40 Set 2

Table of Contents

Lesson 2 Magnification by Microscope Lesson 3 Resolution by Microscope Lesson 4 Nuclear Aperture Lesson 5 Bright Field Microscopy Lesson 6 Dark field Microscopy Lesson 7 Phase contrast microscopy Lesson 8 Phase contrast microscopy -2 Lesson 9 Electron Microscopy Lesson 10 TEM Components Lesson 11 TEM Sample Preparation Lesson 12 SEM Scanning Electron Microscope Lesson 13 Fluorescent Microscope-1 Lesson 14 Fluorescent Microscope-2 Lesson 15 Confocal Microscopy Lesson 16 MicrometryIntroduction Lesson 17 Micrometrycalibration Lesson 18 Microtomy-fixation and processing-1 Lesson 19 Microtomy-fixation and processing-2 Lesson 20 Microtomy-fixation and processing-3 Lesson 21 Microtomy-methods of tissue fixation Lesson 26 Microtomy Parts of microtome Lesson 27 Microtomy Microtomy method, Rotary microtome Lesson 28 Cryomicrotome Lesson 29 CryomicrotomeII Lesson 30 CryomicrotomeIII Lesson 31 Vibratome; Advantages Lesson 32 Gel electrophoresis - Introduction Lesson 33 Agarose Gel Lesson 34 Agarose Gel - Rate of Migration of DNA Lesson 35 Electrophoresis Buffer Lesson 36 Agarose Gel - Reagents and supplies Lesson 37 Agarose Gel - Procedure Lesson 38 Agarose Gel - Detection of DNA Lesson 39 Agarose Gel - Staining with Sybr Gold Lesson 40 Photography of DNA in Gel

What is microscope???

• MICROSCOPE: Is an instrument for viewing · objects that are too small to be seen by the · naked or unaided eye. · • A microscope (Greek: mikron = small and · • A microscope (Greek: mikron = small and · skopein = to look). · • MICROSCOPY: The science of investigating · small objects using a microscope · Ocular / Eyepiece · An eyepiece is that part of an optical system, · which is directed to the viewer. It is a · construction of at least one or more lenses. · The function of the eyepiece in a microscope is · to convert the real- enlarged-intermediate- · image from the objective into an enlarged- · virtual-image. · virtual image. · Lens tube The lens tube is connected with the · eyepiece and it´s main task is to hold it. · The diopter adjustment is a control knob on · your binocular. It is designed to let you · compensate for differences between your own · two eyes. Once you set the diopter, then the · two barrels should stay in proper relation.