ZOO101 Lectures 72-103

Feb. 27

Lesson 72

SQ: LQ: How many types of polymerase chain reaction-PCR? LQ: Write a note on types of polymerase chain reaction-PCR?

Types of polymerase chain reaction-PCR

Types

• Real-Time PCR (quantitative PCR or qPCR) • Reverse-

Transcriptase (RT-PCR)

• Multiplex PCR.
• Nested PCR.

Feb. 27

• High Fidelity PCR.
• Fast PCR.
• Hot Start PCR.
• GC-Rich PCR

SQ: LQ: What is Real-time PCR?

MCQs:Real-time PCR also called quantitative PCR (qPCR) is a variant of standard polymerase chain reaction in which amplification and simultaneous quantitation of a target DNA is done in the same PCR machine, using Depending on the available excitation source and detection filters, a variety of fluorescent dyes may be used in qPCR.

SQ: How many types of real-time PCR?

MCQs: Two most commonly use real-time PCR methods uses SYBR green (a dye that binds to double-stranded DNA but not to single-stranded DNA, and, when so bound, fluoresces) and TaqMan probes respectively.