BT301 Final Term MCQs

Virtual University of Pakistan · BT301 Introduction to Biotechnology · 2nd Semester MSc Zoology 2016-18 · MCQs for Finals · (Topics 96-209)

1pBR322 is an example of in ______ constructed cloning vehicle. Intro Extro

Vitro

In-extro

2It is difficult to clone DNA segments longer than about ______ when plasmids are used as the vector 10,000 bp

15,000 bp

20,000 bp 25,000 bp

3Plasmid ______ has a number of characteristics that make it suitable as cloning vehicle.

pBR322

pBS322 pRR322 pBR232

4Over the years numerous different derivatives of _______ have been constructed. pBR232 pRR322

pBR322

pBS322

5pUC19 carrying sites for many different _______ endonucleases addition active moving

restriction

6Charon 4A and 16 Each has the lacZ gene, encoding enzyme βgalactosidase .

7Connecting the two lambda fragments to foreign DNA by using ________ .

DNA ligase

Polymarise RNA Polymarise II

8Packaging in vitro yields about _______ of vector DNA

104 plaques/ µg 105 plaques/ µg

106 plaques/ µg

107 plaques/ µg

9Plasmids have been constructed which contain a fragment of ʎ DNA including _____ .

Sin site

cos site

tan site

10Cosmids are particularly attractive vectors for constructing libraries of _______ fragments .

eukaryotic genomic

prokaryotic genomic phynotype

11Modified schemes for cloning in Cosmid vectors devised by Ish-Horowicz and Burke in __________

1980

1981

1982 1983 12Phasmid vectors are ____________ of plasmid and ʎ phage sequences .

Separation

Combination

Addition Davison

13Bacterial artificial chromosomes are simply plasmids designed for the cloning of very long segments of DNA ______________.

200,000 to 300,000 bp

100,000 to 300,000 bp

150,000 to 400,000 bp 160,000 to 350,000 bp

14Yeast artificial chromosomes have sites where very ____________ fragments can be inserted

Large RNA Messenger RNA

Large DNA

Small DNA

15YACs with inserts more than ________ are nearly as stable as normal cellular chromosomes

140,000 bp 130,000 bp