BT301 Final Term Slides 96-209

Use of pSC101 for cloning  Expression of Staphylococcus plasmid genes in E. coli pSC101 contain Tetracycline resistance P1248 from Staphylococcus  pBR322 is an example of in vitro constructed cloning vehicle  pBR322 contains the ApR and TcR genes of RSF2124 and pSC101, combined with replication element of pMB1

The origin of plasmid pBR322  It has 40 target sites for different restriction enzymes Improved vectors derived from pBR322  Over the years numerous different derivatives of pBR322 have been constructed  pBR325 encodes chloramphenicol resistance in addition to ampicillin and tetracycline Improved vectors derived from pBR322

pUC19 carrying sites for many different restriction endonucleases  pAT153 another derivative of pBR322 Runaway plasmid vectors  Loss of copy number control –runaway replication Runaway plasmid vectors  Loss of copy number control –runaway replication

vector  Bacteriophage ʎ has a very efficient mechanism for delivering its 48,502 bp of DNA into bacterium  About one-third of the ʎ genome is nonessential and can be replaced with foreign DNA Map of ʎ chromosome